Abstract

View Abstract PDF

Alzheimer‚Äôs disease is very common in America and is partly associated with dysregulation of intracellular trafficking between the endosome and the Golgi. Myosin 2 is involved in intracellular transport among the Golgi, Endosome, and transport vacuole. Previously, we identified that Myo2 plays a role in proper transport of Vps10 and Snc1 to the Golgi. When Myo2 function is mutated, these cargoes are found to be located either at the endosome or the vacuole, suggesting Myo2‚Äôs role in the sorting and delivery of cargo. Despite these results, there was uncertainty for whether the location of Vps10 was in a vacuole or the endosome. We currently examined the precise subcellular localization of Vps10 in WT and myo2 mutant strains. 

Furthermore, we have worked to see if Myo2 is implicated in Snc1 exocytosis to the plasma membrane and found that Snc1 is normally targeted to the plasma membrane even in a myo2 mutant strain. However, we will test this traffic using more myo2 mutants. Our approach to test Snc1’s plasma membrane targeting involves the use of Snc1-pm mutant, an exocytosis competent and endocytosis incompetent mutant. Taken together, our results will shed new light in activities of myo2 in mediating these cargo trafficking toward the Golgi.